Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000.

Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 μg of TCP1 epsilon antibody [GT639] (GTX631668).
Western blot analysis was performed using TCP1 epsilon antibody [GT639] (GTX631668).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

TCP1 epsilon antibody [GT639] detects TCP1 epsilon protein at centrosome and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TCP1 epsilon stained by TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.