GeneTex
United States (US)

TCR gamma + delta antibody [5A6.E91] (FITC)

Orthogonal
GTX15596_94_FACS_19011519_725.jpg

Cat No. GTX15596

Host Mouse
Clonality Monoclonal
Clone Name 5A6.E91
Isotype IgG1
Application ICC/IF, FACS, ELISA, IHC, Neut
Reactivity Human, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS 1-2 μg/test
ELISA Assay dependent
IHC Assay dependent
Neut Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, glycerol, 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

0.10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Pan TCR gamma delta

Purification

Protein A purified

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangement of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia. [provided by RefSeq]
DATA IMAGES
GTX15596_94_FACS_19011519_725.jpg

GTX15596 FACS Image

Orthogonal

FACS analysis of TCR gamma/delta on TCR gamma/delta positive PEER cells(left panel) or TCR gamma/delta negative Jurkat cells(right panel) using GTX15596 TCR gamma + delta antibody [5A6.E91] (FITC).
Dilution : 5ul of primary antibody were used per test

Package List Price ($)
$ 319