TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TEF1 protein stained by TEF1 antibody [GT13112] (GTX632126) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.

Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 μg of TEF1 antibody [GT13112] (GTX632126).
Western blot analysis was performed using TEF1 antibody [GT13112] (GTX632126).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.