GeneTex

  • Country / Location Selection

United States (US)

TERT antibody [2C4]

Anti-TERT antibody [2C4] used in IHC (Paraffin sections) (IHC-P). GTX30410
Anti-TERT antibody [2C4] used in Western Blot (WB). GTX30410
Anti-TERT antibody [2C4] used in IHC (Paraffin sections) (IHC-P). GTX30410

Cat. No. GTX30410

Reference 9

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C4

Isotype

 IgM

Application

 WB, ICC/IF, IHC-P, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($449)
See all TERT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:50-1:200
IHC-P 1:50
FACS 1:50-1:200
IP 1:10 - 1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human Telomerase reverse transcriptase from insect cells. [UniProt# O14746].

Purification

IgM fraction

Conjugation

Unconjugated

RRID

AB_370403

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

telomerase reverse transcriptase , CMM9 , DKCA2 , DKCB4 , EST2 , PFBMFT1 , TCS1 , TP2 , TRT , hEST2 , hTRT

Cellular Localization

nucleoplasm,Nucleus,Cytoplasm,Nucleolus

Background

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TERT antibody [2C4] used in IHC (Paraffin sections) (IHC-P). GTX30410

GTX30410 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX30410 TERT antibody [2C4].

Anti-TERT antibody [2C4] used in Western Blot (WB). GTX30410

GTX30410 WB Image

WB analysis of MJ90 cell lysate using GTX30410 TERT antibody [2C4].
Dilution : 1:500

Anti-TERT antibody [2C4] used in IHC (Paraffin sections) (IHC-P). GTX30410

GTX30410 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX30410 TERT antibody [2C4].

REFERENCE

Application Reference

Min S et al. Cells 2022; 11 (13) Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection.
Mushtaq I et al. Front Oncol 2022; 12 : 919351 Telomere Attrition With Concomitant hTERT Overexpression Involved in the Progression of Gastric Cancer May Have Prognostic and Clinical Implications in High-Risk Population Group From North India.
Application : IHC-P
Reactivity : Human
Haraguchi K et al. Int J Clin Exp Pathol 2022; 15 (1) : 29-37 Human telomerase reverse transcriptase protein expression is associated with survival in patients with oral squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Shin WH et al. Cell Death Dis 2020; 11 (6) : 425 Human telomerase reverse transcriptase positively regulates mitophagy by inhibiting the processing and cytoplasmic release of mitochondrial PINK1.
Shen Y et al. IUBMB Life 2018; (Epub) Telomerase reverse transcriptase suppression inhibits cell proliferation and promotes cell apoptosis in hepatocellular cancer.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

There are currently no reviews for TERT antibody [2C4] (GTX30410). Be the first to share your experience with this product.
Submit a Review
Application Reference
Min S et al. Cells 2022; 11 (13) Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection.
Mushtaq I et al. Front Oncol 2022; 12:919351 Telomere Attrition With Concomitant hTERT Overexpression Involved in the Progression of Gastric Cancer May Have Prognostic and Clinical Implications in High-Risk Population Group From North India.
Application : IHC-P
Reactivity : Human
Haraguchi K et al. Int J Clin Exp Pathol 2022; 15 (1):29-37 Human telomerase reverse transcriptase protein expression is associated with survival in patients with oral squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Shin WH et al. Cell Death Dis 2020; 11 (6):425 Human telomerase reverse transcriptase positively regulates mitophagy by inhibiting the processing and cytoplasmic release of mitochondrial PINK1.
Shen Y et al. IUBMB Life 2018; (Epub) Telomerase reverse transcriptase suppression inhibits cell proliferation and promotes cell apoptosis in hepatocellular cancer.
Application : WB
Reactivity : Human
Haraguchi K et al. APMIS 2017; 125 (9):797-807 The methylation status and expression of human telomerase reverse transcriptase is significantly high in oral carcinogenesis.
Application : IHC-P
Reactivity : Human
Aras G et al. Anticancer Res 2013; 33 (6):2643-50 Immunohistochemical expression of telomerase in patients with non-small cell lung cancer: prediction of metastasis and prognostic significance.
Application : IHC-P
Reactivity : Human
Schauer IG et al. Neoplasia 2013; 15 (4):409-20 Interleukin-1β promotes ovarian tumorigenesis through a p53/NF-κB-mediated inflammatory response in stromal fibroblasts.
Application : WB
Reactivity : Human
Liu S et al. Mol Cancer Ther 2010; 9 (7):2016-25 Telomerase as an important target of androgen signaling blockade for prostate cancer treatment.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 449