APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide of 28 amino acid residues corresponding to CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the C-terminus of human pS2.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCEI , D21S21 , HP1.A , HPS2 , TFF1 , pNR2 , pS2 , trefoil factor 1 , Trefoil factor 1 , Estrogen Inducible Protein pS2
Cellular Localization
Secreted
Background
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-TFF1 antibody [SPM573] used in IHC (Paraffin sections) (IHC-P). GTX35099](/templates/default/js/lightbox/images/loading.gif "Anti-TFF1 antibody [SPM573] used in IHC (Paraffin sections) (IHC-P). GTX35099")
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GTX35099 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX35099 TFF1 antibody [SPM573].
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REFERENCE
There are currently no references for TFF1 antibody [SPM573] (GTX35099). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TFF1 antibody [SPM573] (GTX35099). Be the first to share your experience with this product.
![Anti-TFF1 antibody [SPM573] used in IHC (Paraffin sections) (IHC-P). GTX35099](/upload/website/prouct_img/small/GTX35099/GTX35099_20200115_IHC-P_831_w_23060801_786.webp "Anti-TFF1 antibody [SPM573] used in IHC (Paraffin sections) (IHC-P). GTX35099")
![Anti-TFF1 antibody [SPM573] used in IHC (Paraffin sections) (IHC-P). GTX35099](/upload/website/prouct_img/normal/GTX35099/GTX35099_20200115_IHC-P_831_w_23060801_786.webp "Anti-TFF1 antibody [SPM573] used in IHC (Paraffin sections) (IHC-P). GTX35099")