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TFF1 antibody

Orthogonal Overexpression
Anti-TFF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX121461
Anti-TFF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX121461
Anti-TFF1 antibody used in Western Blot (WB). GTX121461
Anti-TFF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121461
Anti-TFF1 antibody used in Western Blot (WB). GTX121461

Cat. No. GTX121461

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TFF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

9 kDa. ( Note )

Positive Control

TFF1-transfected 293T , MCF-7

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human TFF1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

trefoil factor 1 , BCEI , D21S21 , HP1.A , HPS2 , pNR-2 , pS2

Cellular Localization

Secreted

Background

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TFF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX121461

GTX121461 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Trefoil factor 1(GTX121461) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TFF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX121461

GTX121461 IHC-P Image

Trefoil factor 1 antibody detects Trefoil factor 1 protein at cytoplasm in mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Trefoil factor 1 antibody (GTX121461) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TFF1 antibody used in Western Blot (WB). GTX121461

GTX121461 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF1 antibody (GTX121461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TFF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121461

GTX121461 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Trefoil factor 1(GTX121461) antibody at 1:500 dilution.

Anti-TFF1 antibody used in Western Blot (WB). GTX121461

GTX121461 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF1 antibody (GTX121461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Matsubara D et al. Cancer Sci 2020; 111 (6) : 2183-2195 Reciprocal expression of trefoil factor-1 and thyroid transcription factor-1 in lung adenocarcinomas.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169