APPLICATION
Application Note
Immunohistochemistry 1:25-1:50. This antibody can be used on both frozen and formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution of 1:25-1:50. For paraffin sections, incubation of primary antibody for one hour at 37º is recommended. The optimum conditions should be determined by the individual laboratory.
Calculated MW
Positive Control
About 70% of breast carcinomas show partial staining of tumor cells.
Specificity/Sensitivity
This antibody is specific to pS2 protein, also known as pNR-2.
PROPERTIES
Form
Liquid
Buffer
serum with 0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Antigen Species
Human
Immunogen
Synthetic peptide representing a highly antigenic site near the C-terminus of the pS2 protein.
Purification
Whole antiserum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Trefoil Factor 1,Bcei,D21S21,Hp1.A,Hps2,Pnr-2,Ps2,Tff1
Cellular Localization
Secreted
Background
This antigen was identified by differential screening of cDNA libraries from estrogen responsive breast cancer cell lines. This antigen is expressed in normal gastric and small intestinal mucosa and breast epithelium. In cancer, this antigen is expressed in gastric carcinoma and gynecological cancers. This antibody recognizes cytoplasm with perinuclear condensation. Presence of this antigen in breast carcinomas provides research investigators supplementary information for identifying patients with likely positive response to hormone therapy and a favorable prognosis.
Database
REFERENCE
