A549 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta Receptor I antibody (GTX134209) diluted at 1:1000 and competitor's antibody (ab31013) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HepG2 whole cell and cytoplasmic extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta Receptor I antibody (GTX134209) diluted at 1:1000 and competitor's antibody (ab31013) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.