GeneTex
United States (US)

TGF beta Receptor I antibody

Comparison Orthogonal
GTX134209_42977_20170915_WB_Fraction_competitor_watermark_18121410_572.jpg
GTX134209_42977_20170929_WB_Fraction_competitor_watermark_18121410_630.jpg

Cat No. GTX134209

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

A549 , A549 membrane extract , HepG2 , HepG2 cytoplasmic extract

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.39mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TGF beta Receptor I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AAT5 antibody , ACVRLK4 antibody , ALK5 antibody , LDS1A antibody , LDS2A antibody , MSSE antibody , SKR4 antibody , TGFBR1 antibody , TGFR-1 antibody , TGFR1 antibody , transforming growth factor beta receptor I antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES
GTX134209_42977_20170915_WB_Fraction_competitor_watermark_18121410_572.jpg

GTX134209 WB Image

Comparison Orthogonal

A549 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta Receptor I antibody (GTX134209) diluted at 1:1000 and competitor's antibody (ab31013) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX134209_42977_20170929_WB_Fraction_competitor_watermark_18121410_630.jpg

GTX134209 WB Image

Comparison Orthogonal

HepG2 whole cell and cytoplasmic extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta Receptor I antibody (GTX134209) diluted at 1:1000 and competitor's antibody (ab31013) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf