THP-1 (monocyte; acute monocytic leukemia)
Human THP-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. The THP-1 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 THP-1 cells. A follow-up heat dehydration process was done to ensure the attachment of THP-1 cells and the stability of cellular proteins.