Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE2 antibody (GTX107505) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the Oncotarget in 2018. PMID: 29435158