APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
EL4 Cell Slide(GTX25544) , EL4 Cell Lysate(GTX27183)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TIF1 alpha antibody was raised against a 19 amino acid peptide near the center of human TIF1 alpha.The immunogen is located within amino acids 530 - 580 of TIF1 alpha.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tripartite motif containing 24 , PTC6 , RNF82 , TF1A , TIF1 , TIF1A , TIF1ALPHA , hTIF1
Cellular Localization
Nucleus,Cytoplasm
Background
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31544 WB Image
WB analysis of A431 cell lysate using GTX31544 TIF1 alpha antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX31544 ICC/IF Image
ICC/IF analysis of EL4 cells using GTX31544 TIF1 alpha antibody.
Working concentration : 20 μg/ml
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GTX31544 ICC/IF Image
ICC/IF analysis of EL4 cells using GTX31544 TIF1 alpha antibody.
Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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