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TIF1 alpha antibody

Anti-TIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31544
Anti-TIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31544
Anti-TIF1 alpha antibody used in Western Blot (WB). GTX31544

Cat No. GTX31544

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

EL4 Cell Slide(GTX25544) , EL4 Cell Lysate(GTX27183)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TIF1 alpha antibody was raised against a 19 amino acid peptide near the center of human TIF1 alpha.The immunogen is located within amino acids 530 - 580 of TIF1 alpha.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tripartite motif containing 24 , PTC6 , RNF82 , TF1A , TIF1 , TIF1A , TIF1ALPHA , hTIF1

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-TIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31544

GTX31544 ICC/IF Image

ICC/IF analysis of EL4 cells using GTX31544 TIF1 alpha antibody.
Working concentration : 20 μg/ml

Anti-TIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31544

GTX31544 ICC/IF Image

ICC/IF analysis of EL4 cells using GTX31544 TIF1 alpha antibody.
Working concentration : 2.5 μg/ml

Anti-TIF1 alpha antibody used in Western Blot (WB). GTX31544

GTX31544 WB Image

WB analysis of A431 cell lysate using GTX31544 TIF1 alpha antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE
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Package List Price ($)
$ 329