TIP30 antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 μg hela whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 μg of TIP30 antibody (GTX100119) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody (GTX100119) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
12% SDS PAGE
GTX100119 diluted at 1:5000

TIP30 antibody detects TIP30 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
TIP30 antibody (GTX100119) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TIP30 antibody detects TIP30 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
TIP30 antibody (GTX100119) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TIP30(GTX100119) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using TIP30(GTX100119) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min