Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TMEM66 antibody (GTX119858) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using Transmembrane protein 66(GTX119858) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Transmembrane protein 66(GTX119858) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min