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TMEM66 antibody

Anti-TMEM66 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119858
Anti-TMEM66 antibody used in IHC (Paraffin sections) (IHC-P). GTX119858
Anti-TMEM66 antibody used in Western Blot (WB). GTX119858

Cat No. GTX119858

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMEM66. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731365

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

store-operated calcium entry associated regulatory factor , FOAP-7 , HSPC035 , TMEM66 , XTP3

Cellular Localization

Membrane; Multi-pass membrane protein

Database

DATA IMAGES

Anti-TMEM66 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119858

GTX119858 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Transmembrane protein 66(GTX119858) antibody at 1:500 dilution.

Anti-TMEM66 antibody used in IHC (Paraffin sections) (IHC-P). GTX119858

GTX119858 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Transmembrane protein 66(GTX119858) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMEM66 antibody used in Western Blot (WB). GTX119858

GTX119858 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TMEM66 antibody (GTX119858) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169