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TMPRSS2 antibody [N2C3]

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

Cat No. GTX100743

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($379),
25 μl ($169)
See all TMPRSS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMPRSS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952298

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transmembrane serine protease 2 , PP9284 , PRSS10

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Transmembrane protease , serine 2 catalytic chain: Secreted

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743

GTX100743 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 26018085

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Hsin Yeh et al. Phytomedicine 2022; 103 : 154215 GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus.
Ko Sato et al. J Virol 2021; 95 (21) : e0129621 TMPRSS2 Activates Hemagglutinin-Esterase Glycoprotein of Influenza C Virus.
Generali M et al. J Cell Mol Med 2021; Heterogeneous expression of ACE2 and TMPRRS2 in mesenchymal stromal cells.
Application : WB
Yamada T. et al. Nat Immunol 2021; (Epub) RIG-I triggers a signaling-abortive anti-SARS-CoV-2 defense in human lung cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169
Currently in production