TMPRSS2 antibody [N2C3]

Name: TMPRSS2 antibody [N2C3]
Brand: GeneTex
SKU: GTX100743
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

Cat. No. GTX100743

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all TMPRSS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMPRSS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952298

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transmembrane serine protease 2 , PP9284 , PRSS10

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Transmembrane protease , serine 2 catalytic chain: Secreted

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 7113 TMPRSS2
UniProt: O15393 TMPRSS2

Research Area

DATA IMAGES

GTX100743 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Hsin Yeh et al. Phytomedicine 2022; 103 : 154215 GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus.

Application : WB

Reactivity : Human

Ko Sato et al. J Virol 2021; 95 (21) : e0129621 TMPRSS2 Activates Hemagglutinin-Esterase Glycoprotein of Influenza C Virus.

Application : ICC/IF

Reactivity : Human

Generali M et al. J Cell Mol Med 2021; Heterogeneous expression of ACE2 and TMPRRS2 in mesenchymal stromal cells.

Application : WB

Yamada T. et al. Nat Immunol 2021; (Epub) RIG-I triggers a signaling-abortive anti-SARS-CoV-2 defense in human lung cells.

Application : WB

Reactivity : Human

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