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TMPRSS2 antibody [N2C3]

Orthogonal
Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743

Cat. No. GTX100743

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TMPRSS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

LNCap

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human TMPRSS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952298

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane serine protease 2 , PP9284 , PRSS10

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Transmembrane protease , serine 2 catalytic chain: Secreted

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743

GTX100743 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743

GTX100743 WB Image

The data was published in the 2022 in J Cell Mol Med. PMID: 34821008

REFERENCE

Application Reference

Chen YR et al. Int J Mol Sci 2023; 24 (20) Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
Chien LH et al. Biomed Pharmacother 2022; 153 : 113434 Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors.
Application : WB
Reactivity : Human
Sun TK et al. Int J Mol Sci 2022; 23 (23) Schizophyllum commune Reduces Expression of the SARS-CoV-2 Receptors ACE2 and TMPRSS2.
Application : WB
Reactivity : Human
Hsin Yeh et al. Phytomedicine 2022; 103 : 154215 GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus.
Application : WB
Reactivity : Human
Ko Sato et al. J Virol 2021; 95 (21) : e0129621 TMPRSS2 Activates Hemagglutinin-Esterase Glycoprotein of Influenza C Virus.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
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Package List Price ($)
$ 399
$ 169