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TMPRSS2 antibody [N2C3]

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743
Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

Cat. No. GTX100743

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMPRSS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952298

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transmembrane serine protease 2 , PP9284 , PRSS10

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Transmembrane protease , serine 2 catalytic chain: Secreted

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TMPRSS2 antibody [N2C3] used in Western Blot (WB). GTX100743

GTX100743 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMPRSS2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100743

GTX100743 IHC-P Image

TMPRSS2 antibody [N2C3] detects TMPRSS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
TMPRSS2 stained by TMPRSS2 antibody [N2C3] (GTX100743) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

TMPRSS2 antibody [N2C3] Cat. No. GTX100743

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in J Cell Mol Med. PMID: 34821008
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169