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United States (US)

TMS1 antibody [N1C3]

Overexpression
GTX102474_40527_20181109_WB_B_19011519_159.jpg
GTX102474_40527_WB_B_19011506_771.jpg
GTX102474_40527_IFA_19011506_343.jpg
GTX102474_40527_20160428_WB_19011506_314.jpg

Cat No. GTX102474

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

THP-1 , 3xFlag-human TMS1-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ASC antibody, CARD5 antibody, MGC10332 antibody, TMS antibody, TMS-1 antibody, TMS1 antibody, PYCARD antibody, target of methylation-induced silencing 1 antibody, apoptosis-associated speck-like protein containing a CARD antibody, caspase recruitment domain-containing protein 5 antibody, PYD and CARD domain containing antibody

Cellular Localization

Cytoplasm

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102474_40527_20181109_WB_B_19011519_159.jpg

GTX102474 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102474_40527_WB_B_19011506_771.jpg

GTX102474 WB Image

Overexpression

TMS1 antibody detects TMS1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of 3xFlag-human TMS1-transfected 293T cells
12 % SDS-PAGE
TMS1 antibody (GTX102474) dilution: 1:20000

GTX102474_40527_IFA_19011506_343.jpg

GTX102474 ICC/IF Image

TMS1 antibody [N1C3] detects TMS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: TMS1 protein stained by TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX102474_40527_20160428_WB_19011506_314.jpg

GTX102474 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169