APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
THP-1 , 3xFlag-human TMS1-transfected 293T
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TMS1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ASC antibody, CARD5 antibody, MGC10332 antibody, TMS antibody, TMS-1 antibody, TMS1 antibody, PYCARD antibody, target of methylation-induced silencing 1 antibody, apoptosis-associated speck-like protein containing a CARD antibody, caspase recruitment domain-containing protein 5 antibody, PYD and CARD domain containing antibody
Cellular Localization
Cytoplasm
Background
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102474 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102474 WB Image
TMS1 antibody detects TMS1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of 3xFlag-human TMS1-transfected 293T cells
12 % SDS-PAGE
TMS1 antibody (GTX102474) dilution: 1:20000
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GTX102474 ICC/IF Image
TMS1 antibody [N1C3] detects TMS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: TMS1 protein stained by TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX102474 WB Image
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.
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