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TNF Receptor I antibody

Cat No. GTX10500

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse
Package
50 μg ($409)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TNF receptor superfamily member 1A , CD120a , FPF , TBP1 , TNF-R , TNF-R-I , TNF-R55 , TNFAR , TNFR1 , TNFR55 , TNFR60 , p55 , p55-R , p60

Cellular Localization

Cell membrane,Golgi apparatus membrane,Secreted

Background

TNF RI (CD120a) is a 55 kDa transmembrane glycoprotein that is expressed by virtually all nucleated mammalian cells. Among the numerous cells known to express TNF RI are hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34+ hematopoietic progenitors. Both TNF-alpha and TNF-beta bind to TNF RI. Soluble TNF-alpha binds with a Kda in the range of 20-60 pM, while TNF-beta binds with a Kda equal to 650 pM. TNF RI relative to TNF RII seems to be the more physiologically-relevant receptor, whereas TNF-R2 appears to play a direct role in only a limited number of TNF responses. Soluble TNF RI, which blocks TNF-alpha activity, has been identified in both urine and blood (1-3 ng/mL). Serum levels of sTNF receptors increase dramatically in certain pathological situations. Two soluble forms have been identified and are believed to be generated by proteolytic cleavage. Human TNF RI has 64% amino acid sequence identity (70% in the extracellular region) with mouseTNF-R1, and human TNF RI binds human and mouse TNF-alpha with equal affinity. The extracellular region has four cysteine-rich motifs, the first of which is suggested to be required for binding. The intracellular portion of TNF R1 contains a 'death domain' of about 70 amino acids that is required for the signaling of apoptosis and NF-kappaB activation. TNF binds to the extracellular domain of TNF R1 and induces receptor trimerization. Then, the aggregated death domain of TNF R1 recruits the adapter protein TRADD. TRADD, in turn, recruits FADD, TRAF2 and RIP to form the TNF R1 signaling complex and activate signaling cascades leading to apoptosis, JNK/SAPK activation, and NF-kappaB activation respectively. However, TNF R1 self-associates and signals independently of ligand when overexpressed. This apparent paradox may be explained by silencer of death domains (SODD), a widely expressed approximately 60 kDa protein that was found to be associated with the death domain of TNF-RI.

Database

Research Area

REFERENCE
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SDS
PBS.pdf
Package List Price ($)
$ 409