• Country / Location Selection

United States (US)

TNF Receptor II antibody [22221]

Cat No. GTX10503

Host Mouse
Clonality Monoclonal
Clone Name 22221
Isotype IgG2a
Application ICC/IF, FACS, Neutralizing/Blocking
Reactivity Human
100 μg ($289)

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 8-25 μg/ml
FACS Assay dependent
Neutralizing/Blocking Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

human peripheral blood lymphocytes


The antibody neutralizes the biological effect of recombinant human TNF RII. It does not neutralize or cross-react with TNF RI.




PBS containing carbohydrates


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species



recombinant human tumor necrosis factor receptor II, expressed in Escherichia coli.


Purified immunoglobulin




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


TNF receptor superfamily member 1B , CD120b , TBPII , TNF-R-II , TNF-R75 , TNFBR , TNFR1B , TNFR2 , TNFR80 , p75 , p75TNFR


TNF RII (p75, CD120b) is a 75 kD transmembrane glycoprotein originally isolated from a human lung fibroblast library. Among the multitude of cells known to express TNFRII are monocytes, endothelial cells, Langerhans cells, and macrophages. Mouse to human amino acid sequence identity in the TNFRII cytoplasmic domain is 73%, while amino acid sequence identity in the extracellular region falls to 58%. This drop in extracellular identity is reflected in the observation that human TNF-alpha is not active in the mouse system. TNF RII to TNF RI, amino acid sequence identity is only about 20% in the extracellular region and 5% in the cytoplasmic domain. TNF RII consists of a 240 amino acid residue extracellular region, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain. TNF R1 and TNF R2 are members of a family of structurally related membrane receptors that includes lymphotoxin receptor, Fas, WSL1, DR4, CD40, CD30, CD27, 41BB, OX40, and p75 nerve growth factor receptor. Members of the TNFR family can interact through their cytoplasmic domains with a range of intracellular signalling proteins, most of which fall into two distinct groups. The first is the death domain containing proteins, including TRADD, FADD/MORT1, and RIP, which associate directly with receptors also containing death domains, such as TNF R1 and Fas. The second is the TRAF proteins. TRAF1 and TRAF2 were originally identified by their association with the cytoplasmic domain of TNFR2. TRAF proteins appear to function as adaptor proteins. TRAF2 directly binds at least eight intracellular molecules, including TRAF1, cIAP1, cIAP2, ITRAF/TANK, A20, TRIP, RIP, and NIK. The best characterized TRAFmediated signal transduction pathway is the activation of NF-B transcription factors. TRAF2 mediates NF-B activation via the recruitment of the serine/threonine kinase NIK, which can in turn activate CHUK, an IBspecific kinase that triggers IB degradation. In addition to recruiting mediators of NF-B activation, TRAF2 can bind at least three other molecules (I-TRAF/TANK, A20, TRIP) that inhibit its ability to activate NF-B.


Research Area

There are currently no references for TNF Receptor II antibody [22221] (GTX10503). Be the first to share your publications with this product.
There are currently no reviews for TNF Receptor II antibody [22221] (GTX10503). Be the first to share your experience with this product.