TNIK antibody detects TNIK protein at cytosol on mouse Colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse Colon
TNIK antibody (GTX13141) dilution: 1:100
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TNIK antibody detects TNIK protein at cytosol on Mouse Kidney by immunohistochemical analysis.
Sample: Paraffin-embedded Mouse Kidney
TNIK antibody (GTX13141) dilution: 1:100
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TNIK antibody validation by shRNA knock-down
Upper panel: TNIK antibody (GTX13141)
Lower panel: p84 antibody(GTX70220)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TNIK shRNA-transfected 293T cells
5% SDS-PAGE
TNIK antibody (GTX13141) dilution: 1:5000
p84 antibody(GTX70220) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TNIK antibody detects TNIK protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNIK protein stained by TNIK antibody (GTX13141) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

TNIK antibody detects TNIK protein at cytosol on Mouse small intestine by immunohistochemical analysis.
Sample: Paraffin-embedded Mouse small intestine
TNIK antibody (GTX13141) dilution: 1:100
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min