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TOMM22 antibody [1C9-2]

Cat No. GTX10436

Host Mouse
Clonality Monoclonal
Clone Name 1C9-2
Isotype IgG1
Application WB, ICC/IF, EM
Reactivity Human, Monkey
Package
50 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0 μg/ml
ICC/IF Assay dependent
EM Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

Raji
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Monkey

Immunogen

membrane fraction from Vero (monkey kidney-derived) cells.[]

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

translocase of outer mitochondrial membrane 22 , 1C9-2 , MST065 , MSTP065 , TOM22

Cellular Localization

Mitochondrion outer membrane

Background

Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100 kDa core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.

Database

Research Area

REFERENCE
REVIEW
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Package List Price ($)
$ 319