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TPH2 antibody

Anti-TPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31130
Anti-TPH2 antibody used in Immunoprecipitation (IP). GTX31130
Anti-TPH2 antibody used in Western Blot (WB). GTX31130

Cat. No. GTX31130

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, Rabbit
Package
50 μl ($399)
See all TPH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Distilled water, 1.0mg/ml BSA

Preservative

0.1% Sodium azide

Storage

Store at 4C. Do not freeze.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the N-terminal region of TPH2: : R(15) R G L S L D S A V P E D H Q L (30)

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Research Area

DATA IMAGES

Anti-TPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31130

GTX31130 ICC/IF Image

Immunofluorescent staining in brain section of wild-type B6 adult mouse (P-45) using anti-TPH2 antibody (GTX31130) at a dilution of 1:100.
Anti-TPH2 antibody used in Immunoprecipitation (IP). GTX31130

GTX31130 IP Image

Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [GTX31130] - Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (GTX31130). GTX31130 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT.

Anti-TPH2 antibody used in Western Blot (WB). GTX31130

GTX31130 WB Image

Western Blot analysis of TPH2 in human brain tissue extracts using anti-TPH2 antibody (GTX31130).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399