GeneTex
United States (US)

TPH2 (phospho Ser19) antibody

TPH2-phospho-Ser19-antibody_Western-blot_GTX31131-1_18121411_298.jpg
TPH2-phospho-Ser19-antibody-GTX31131-WB-1_18121411_479.jpg

Cat No. GTX31131

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Rat
APPLICATION

Calculated MW

55 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADHD7, FLJ37295, MGC138871, TPH2, tryptophan hydroxylase2, tryptophan hydroxylase 2, MGC138872, NTPH, TPH2 (phospho S19), TPH2 (phospho Ser19), TPH2 (pS19), TPH2 (pSer19), TPH2 phospho S19, TPH2 phospho Ser19, Phospho TPH2 (S19), Phospho TPH2 (Ser19), Phospho TPH2 (pS19), Phospho TPH2 (pSer19)

Background

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (5-HT) and might be related to the pathogenesis of major depression (MD). Two isoforms are known, TPH1 and TPH2. . TPH1 is phosphorylated by cAMP-dependent protein kinase A . Recently, it has been shown that expression of the TPH1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. Results indicate that TPH1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. In addition, the results suggest that, in mice, serotonin has differential effects on the pulmonary vasculature and right ventricular hypertrophy.

Database

Research Area

DATA IMAGES
TPH2-phospho-Ser19-antibody_Western-blot_GTX31131-1_18121411_298.jpg

GTX31131 WB Image

Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19 using TPH2 (phospho Ser19) antibody (GTX31131)

TPH2-phospho-Ser19-antibody-GTX31131-WB-1_18121411_479.jpg

GTX31131 WB Image

Western Blot: Tyrosine Hydroxylase (phospho Ser19) Antibody [GTX31131] - Non-phospho-recombinant tyrosine hydroxylase (Lane 1), PC-12 cell lysate (Lane 2), and PC-12 cell lysate stimulated with KCl, 30 mM for 10 min (Lane 3) showing specific immunolabeling of the ~60k tyrosine hydroxylase phosphorylated at Ser19.

Package List Price ($)
$ 329