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TPH2 (phospho Ser19) antibody

Anti-TPH2 (phospho Ser19) antibody used in Western Blot (WB). GTX31131
Anti-TPH2 (phospho Ser19) antibody used in Western Blot (WB). GTX31131

Cat No. GTX31131

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Rat
Package
100 μl ($399)
See all TPH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tryptophan Hydroxylase 2 , Ntph , Tph2

Background

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (5-HT) and might be related to the pathogenesis of major depression (MD). Two isoforms are known, TPH1 and TPH2. . TPH1 is phosphorylated by cAMP-dependent protein kinase A . Recently, it has been shown that expression of the TPH1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. Results indicate that TPH1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. In addition, the results suggest that, in mice, serotonin has differential effects on the pulmonary vasculature and right ventricular hypertrophy.

Database

Research Area

DATA IMAGES

Anti-TPH2 (phospho Ser19) antibody used in Western Blot (WB). GTX31131

GTX31131 WB Image

Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19 using TPH2 (phospho Ser19) antibody (GTX31131)

Anti-TPH2 (phospho Ser19) antibody used in Western Blot (WB). GTX31131

GTX31131 WB Image

Western Blot: Tyrosine Hydroxylase (phospho Ser19) Antibody [GTX31131] - Non-phospho-recombinant tyrosine hydroxylase (Lane 1), PC-12 cell lysate (Lane 2), and PC-12 cell lysate stimulated with KCl, 30 mM for 10 min (Lane 3) showing specific immunolabeling of the ~60k tyrosine hydroxylase phosphorylated at Ser19.

REFERENCE

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SDS
Glycerol.pdf
Package List Price ($)
$ 399