Immunohistochemical analysis of paraffin-embedded human gastric cancer, using TRIM23(GTX100057) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TRIM23 antibody [N1], N-term detects TRIM23 protein at cytoplasm by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23(GTX100057) antibody (Green) at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.