Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM36 antibody [C3], C-term (GTX106290) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A549, using TRIM36(GTX106290) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A549 7.5% SDS PAGE
GTX106290 diluted at 1:1000