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TSG101 antibody [4A10-RB]

Recombinant antibody KO/KD
Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396
Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396
Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396
Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396

Cat. No. GTX635396

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 4A10-RB

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TSG101 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 167-374 of TSG101 protein expressed in E. coli.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor susceptibility 101 , TSG10 , VPS23

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396

GTX635396 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (GTX635396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396

GTX635396 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (GTX635396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396

GTX635396 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (GTX635396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10-RB] used in Western Blot (WB). GTX635396

GTX635396 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (GTX635396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ge Gao et al. Am J Cancer Res 2022; 12 (5) : 1934-1959 Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.
Application : WB
Reactivity : Human
Simsone Z et al. BMC Cancer 2021; 21 (1) : 1087 Cancer microcell initiation and determination.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations