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TSG101 antibody

Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in ELISA (ELISA). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Immunoprecipitation (IP). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX118736

Cat No. GTX118736

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA, sELISA
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX118736 , Detection: GTX70255

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , A549 , H1299 , HCT116 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TSG101. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Susceptibility 101,Tsg10,Vps23,Tsg101

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Late endosome membrane; Peripheral membrane protein

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in ELISA (ELISA). GTX118736

GTX118736 ELISA Image

ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 µg/mL and GTX70255 for detection at a concentration of 1.5 µg/mL.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

TSG101 antibody detects TSG101 protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
TSG101 antibody (GTX118736) dilution: 1:500

Anti-TSG101 antibody used in Immunoprecipitation (IP). GTX118736

GTX118736 IP Image

Immunoprecipitation of TSG101 protein from A549 whole cell extracts using 5 μg of TSG101 antibody (GTX118736).
Western blot analysis was performed using TSG101 antibody (GTX118736).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

TSG101 antibody detects TSG101 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
TSG101 antibody (GTX118736) dilution: 1:500

Anti-TSG101 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118736

GTX118736 ICC/IF Image

TSG101 antibody detects TSG101 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 2% paraformaldehyde at RT for 15 min, and be permeabilized in 0.1% saponin, 10% NGS, 100mM glycine for 30 min.
Green: TSG101 protein stained by TSG101 antibody (GTX118736) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbite IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX118736

GTX118736 IHC-P Image

TSG101 antibody detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody (GTX118736) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169