Talin-1 antibody

Cat. No. GTX134635
Cat. No.  GTX134635
Anti-Talin-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134635
GTX134635 ICC/IF Image

Talin-1 antibody detects Talin-1 protein at cytoplasm and focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Talin-1 stained by Talin-1 antibody (GTX134635) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

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Anti-Talin-1 antibody used in Western Blot (WB). GTX134635
GTX134635 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin-1 antibody (GTX134635) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Talin-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134635
GTX134635 IHC-P Image

Talin-1 antibody detects Talin-1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Talin-1 stained by Talin-1 antibody (GTX134635) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Talin-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134635
Anti-Talin-1 antibody used in Western Blot (WB). GTX134635
Anti-Talin-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134635
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human, Mouse
Summary
Talin antibody recognizes the cytoskeletal component talin, a ~270 kDa protein involved in cell mechanotransduction that links the cytoskeleton to integrins. It forms an antiparallel homodimer and interacts with vinculin, actin, and integrins. Talin is incorporated very early in the cell-extracellular matrix (ECM) adhesion process.
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