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Talin antibody [8D4]

Cat. No. GTX11188

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8D4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, IP, Immunoassay

Reactivity

 Human, Mouse, Rat, Chicken, Gerbil, Xenopus
Package
100 μl ($519)
See all Talin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100
ICC/IF 1:500
IHC-Fr Assay dependent
IP Assay dependent
Immunoassay Assay dependent
Not tested in other applications.

Calculated MW

272 kDa. ( Note )

Positive Control

chicken gizzard, Rat2

Product Note

Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Chicken

Immunogen

purified chicken gizzard talin.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_373100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

talin 1 , talin-1

Cellular Localization

Cytoplasm, cytoskeleton

Background

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.

Database

Research Area

REFERENCE

Application Reference

Xie Y et al. Anal Biochem 2019; 577 : 1-13 Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions.
Application : IHC-Fr
Reactivity : Mouse
Miyoshi H et al. Anal Sci 2016; 32 (11) : 1207-1211 A Perturbation Analysis to Understand the Mechanism How Migrating Cells Sense and Respond to a Topography in the Extracellular Environment.
Application : ICC/IF
Reactivity : Gerbil

Clone Reference

Hongchi Yu et al. Int J Mol Med . 2018; 41 (5):2573-2588 Inhibition of cell migration by focal adhesion kinase: Time-dependent difference in integrin-induced signaling between endothelial and hepatoblastoma cells
Application : WB, ICC/IF
Reactivity : Human
Terhi P Teräväinen et al. PLoS One. 2013; 8 (8) αV-integrins are required for mechanotransduction in MDCK epithelial cells
Application : WB
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 519