ICC/IF analysis of SH-SY5Y cells using GTX24749 Tau (phospho Ser199) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton„ X-100 for 10 minutes

IHC-P analysis of mouse brain tissue using GTX24749 Tau (phospho Ser199) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

WB (peptide competition) analysis of human recombinant Tau untreated (Lane 1) or treated with GSK-3 beta (1 μg per μg Tau) for 45 minutes (Lanes 2-5) using GTX24749 Tau (phospho Ser199) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

IHC-P analysis of human brain tissue using GTX24749 Tau (phospho Ser199) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100