Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at nucleus and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (GTX135775) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (GTX135775) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (GTX135775) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (GTX135775) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min