Indirect ELISA analysis performed by coating plate with recombinant Tet Repressor protein (162.21-2.53 nM). Coated protein was probed with Tet Repressor antibody (GTX70489) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (tetR) antibody (GTX131984) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Detection of Tet- Repressor (TetR) using anti-TetR (GTX70489) at a 1:1000 dilution.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.