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ThrRS antibody [N3C3]

Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359
Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359
Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359
Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359

Cat. No. GTX116359

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ThrRS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

A549 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ThrRS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617685

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

threonyl-tRNA synthetase , ThrRS

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359

GTX116359 WB Image

ThrRS antibody detects ThrRS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ThrRS antibody (GTX116359) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359

GTX116359 IHC-P Image

ThrRS antibody [N3C3] detects ThrRS protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ThrRS antibody [N3C3] (GTX116359) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359

GTX116359 IHC-P Image

ThrRS antibody [N3C3] detects ThrRS protein at cytoplasm on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ThrRS antibody [N3C3] (GTX116359) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359

GTX116359 WB Image

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX116359 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Mirando AC et al. Methods 2017; 113 : 132-138 Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169