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ThrRS antibody [N3C3]

Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359
Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359
Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359
Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359

Cat No. GTX116359

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ThrRS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617685

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

threonyl-tRNA synthetase , ThrRS

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family [provided by RefSeq]

Database

DATA IMAGES

Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359

GTX116359 WB Image

ThrRS antibody detects ThrRS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ThrRS antibody (GTX116359) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359

GTX116359 IHC-P Image

ThrRS antibody [N3C3] detects ThrRS protein at cytoplasm on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ThrRS antibody [N3C3] (GTX116359) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ThrRS antibody [N3C3] used in Western Blot (WB). GTX116359

GTX116359 WB Image

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX116359 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ThrRS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116359

GTX116359 IHC-P Image

ThrRS antibody [N3C3] detects ThrRS protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ThrRS antibody [N3C3] (GTX116359) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169