Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 1 antibody [HL3593] (GTX641564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 1 antibody [HL3593] (GTX641564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 1 antibody [HL3593] (GTX641564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.