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Thymidylate synthase antibody

Anti-Thymidylate synthase antibody used in  (). GTX27398

Cat No. GTX27398

Host

Sheep

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat, Monkey, E. coli
Package
1 mg ($299)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Product Note

Detects Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain non-proliferating cells.Normal colon mucosa shows weak staining. However, some colorectal cancer specimens show very strong staining.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium phosphate, 0.15 M sodium chloride, pH 7.2, containing 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Thymidylate synthase (36 kDa) produced in E.coli .

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thymidylate synthetase , HST422 , TMS , TS

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Mitochondrion matrix,Mitochondrion inner membrane

Background

Because inhibition of Thymidylate Synthase (TS) prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors.

Database

Research Area

DATA IMAGES

Anti-Thymidylate synthase antibody used in  (). GTX27398

GTX27398 Image

Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 μg of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-anti-Sheep IgG diluted 1:1,000 and color development using TMB (TMBM-100) substrate.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 299