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Thyroxine Binding Globulin antibody

Anti-Thyroxine Binding Globulin antibody used in IHC (Paraffin sections) (IHC-P). GTX109010
Anti-Thyroxine Binding Globulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109010
Anti-Thyroxine Binding Globulin antibody used in Western Blot (WB). GTX109010

Cat No. GTX109010

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thyroxine Binding Globulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951866

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family A member 7 , TBG , TBGQTL

Cellular Localization

Secreted

Background

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3 -triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.

Database

Research Area

DATA IMAGES

Anti-Thyroxine Binding Globulin antibody used in IHC (Paraffin sections) (IHC-P). GTX109010

GTX109010 IHC-P Image

Thyroxine Binding Globulin antibody detects SERPINA7 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Thyroxine Binding Globulin antibody (GTX109010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Thyroxine Binding Globulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109010

GTX109010 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBG(GTX109010) antibody at 1:200 dilution.

Anti-Thyroxine Binding Globulin antibody used in Western Blot (WB). GTX109010

GTX109010 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX109010 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169