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Topoisomerase I antibody [Mab1]

Cat No. GTX12407

Host

Mouse

Clonality

Monoclonal

Clone Name

Mab1

Isotype

IgG1

Application

WB

Reactivity

Human
Package
50 μl ($279)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

A231

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

purified human topoisomerase I

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA topoisomerase I , TOPI

Cellular Localization

nucleoplasm,Nucleolus

Background

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single-stranded nick which is then re-ligated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. In normal eukaryotic cells, DNA topoisomerase I does not show relevant fluctuations across the cell cycle, unlike DNA topoisomerase II alpha. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti-tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by 32P transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability. Renal tumors were found not to show any differential levels compared with normal kidney. Topo I may be useful for the evaluation of DNA topoisomerase I expression in normal tissues, solid tumors and in further studies of ovarian, colorectal, cervical and prostatic tumors.

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 279