Analysis of Protein Array containing more than 19,000 full-length human proteins using Topoisomerase II alpha Mouse Monoclonal Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

IHC-P analysis of human tonsil tissue using GTX35138 Topoisomerase II alpha antibody [TOP2A/1362].

IHC-P analysis of human bladder carcinoma tissue using GTX35138 Topoisomerase II alpha antibody [TOP2A/1362].

WB analysis of HeLa cell lysate using GTX35138 Topoisomerase II alpha antibody [TOP2A/1362].

ICC/IF analysis of HeLa cells using GTX35138 Topoisomerase II alpha antibody [TOP2A/1362].
Green : Primary antibody
Red : nucleus