Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 μg of Topoisomerase II beta antibody (GTX102640) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (GTX102640) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 μg of whole cell lysate)
A: Jurkat
5% SDS PAGE
GTX102640 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Topoisomerase II beta(GTX102640) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Biomolecules in 2019. PMID: 31505885