Wild-type (WT) and Transgelin 2 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Transgelin 2 antibody detects Transgelin 2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody (GTX115082) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115082 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.