Room temperature. If stored at 2-8ºC, some sodium chloride will be precipitated, if this occurs, bring the buffer to room temperature and mix well till all sodium chloride is dissolved.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
This buffer is supplied as 10X TBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any phosphate, sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase, and antibodies to phosphoproteins. This buffer does not contain any proteins.