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Trident 10X TBST

Cat No. GTX30976

Reference 2

Application

 WB, ELISA
Package
100 ml ($59)
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PRODUCT

Summary

Tris-Buffered Saline with Tween Solution (10X)

APPLICATION

Application Note

Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8ºC for several weeks. Wash membrane or ELISA plates for 10 minutes each for 3 times. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.

PROPERTIES

Form

Liquid

Storage

Room temperature. If stored at 2-8ºC, some sodium chloride will be precipitated, if this occurs, bring the buffer to room temperature and mix well till all sodium chloride is dissolved.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

This buffer is supplied as 10X TBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any phosphate, sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase, and antibodies to phosphoproteins. This buffer does not contain any proteins.

REFERENCE

Application Reference

Mason DE et al. J Cell Biol 2019; (Epub) YAP and TAZ limit cytoskeletal and focal adhesion maturation to enable persistent cell motility.
Mason D.E. et al. bioRxiv 2018; (Epub) Persistent cell motility requires transcriptional feedback of cytoskeletal – focal adhesion equilibrium by YAP/TAZ.
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Package List Price ($)
$ 59