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Trident Endosome Isolation Kit

Trident Endosome Isolation Kit. GTX35192

Cat No. GTX35192

Application WB
Package
20 test ($399),
5 test ($189)
PRODUCT

Product Description

Early endosomes (EE) provide the starting point for late endosome maturation. The EEs are mainly derived from primary endocytic vesicles that fuse with each other. EEs receive endocytic cargo not only through the clathrin-mediated pathway but several other pathways. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires large amount of starting material and the methods are tedious and time consuming. The Endosome Isolation and Cell Fractionation Kit provides a spin-column based novel endosome isolation technology that is rapid, simple and requires smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. The availability of the kit should facilitate the research in the field.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Storage

Ship at ambient temperature and store the kit at 4ºC

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Spin column-based protein extraction and cell fractionation technologies were developed by Invent Biotechnologies, Inc.
DATA IMAGES
Trident Endosome Isolation Kit. GTX35192

GTX35192 WB Image

HepG2 cells were lysed and extracted by GTX35192 Endosome Isolation and Cell Fractionation Kit.
When following the protocol, cell extracts would be isolated into cytosolic and total membrane.
The fractions were then assayed by Western Blot analysis, membrane was blotted with an early endosome antigen 1- EEA1 antibody (GTX109638, subcellular marker), a plasma membrane protein- Na/K ATPase antibody (subcellular marker), and a nucleolar small nuclear ribonucleoprotein- Fibrillarin antibody (GTX113684, subcellular marker).
Abbreviations:
WCE : whole cell extract
CE : cytosolic extract

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Package List Price ($)
$ 399
$ 189