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Trident Endosome Isolation Kit

Orthogonal
Trident Endosome Isolation Kit. GTX35192

Cat. No. GTX35192

Reference 3

Application

 WB
Package
20 test ($599),
5 test ($269)
PRODUCT

Summary

Early endosomes (EE) provide the starting point for late endosome maturation. The EEs are mainly derived from primary endocytic vesicles that fuse with each other. EEs receive endocytic cargo not only through the clathrin-mediated pathway but several other pathways. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires large amount of starting material and the methods are tedious and time consuming. The Endosome Isolation and Cell Fractionation Kit provides a spin-column based novel endosome isolation technology that is rapid, simple and requires smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. The availability of the kit should facilitate the research in the field.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Ship at ambient temperature and store the kit at 4ºC.

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Spin column-based protein extraction and cell fractionation technologies were developed by Invent Biotechnologies, Inc.

DATA IMAGES

Trident Endosome Isolation Kit. GTX35192

GTX35192 WB Image

Orthogonal

HepG2 cells were lysed and extracted by GTX35192 Endosome Isolation and Cell Fractionation Kit.
When following the protocol, cell extracts would be isolated into cytosolic and total membrane.
The fractions were then assayed by Western Blot analysis, membrane was blotted with an early endosome antigen 1- EEA1 antibody (GTX109638, subcellular marker), a plasma membrane protein- Na/K ATPase antibody (subcellular marker), and a nucleolar small nuclear ribonucleoprotein- Fibrillarin antibody (GTX113684, subcellular marker).
Abbreviations:
WCE : whole cell extract
CE : cytosolic extract

REFERENCE

Application Reference

M Lucarelli et al. Mol Neurobiol 2023; Reduced Cerebellar BDNF Availability Affects Postnatal Differentiation and Maturation of Granule Cells in a Mouse Model of Cholesterol Dyshomeostasis
Fang Y et al. J Clin Invest 2021; 131 (1) MAL2 drives immune evasion in breast cancer by suppressing tumor antigen presentation.
Lim TJF et al. J Exp Med 2020; 217 (8) Talin1 controls dendritic cell activation by regulating TLR complex assembly and signaling.
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Package List Price ($)
$ 599
$ 269