PRODUCT
Summary
GeneTex Mitochondria Isolation Kit (For Mammalian Cells And Tissues) is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the protein isolation procedure is simple, easy and user friendly with high yield. Unlike many commercial mitochondria preparation kits, this kit offers wide range of starting cells (5-50 million/sample) and intact mitochondria are isolated. The buffers are detergent and EDTA free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in less than 30 min.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Product Note
This product is not recommended for muscle samples.
PROPERTIES
Storage
Store Buffer A and Buffer B at -20ºC upon arrival.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
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GTX35191 WB Image
HepG2 cells were lysed and extracted by GTX35191 Mitochondria Isolation Kit . When following the protocol, cell extracts would be isolated into cytosolic and total membrane. The fractions were then assayed by Western Blot analysis, membrane was blotted with an organelle membrane protein- ATP5D antibody (GTX101503, subcellular marker) and beta Catenin antibody (GTX101435, internal control). Abbreviations: WCE : whole cell extract CE : cytosolic extract ME: total membrane extract
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REFERENCE
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REVIEW
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