United States (US)

Trident Mitochondria Isolation Kit

GTX35191_20180725_WB_Mitochondria Isolation Kit_18121410_388.jpg

Cat No. GTX35191

Application WB, IP, ELISA, Enzyme Assay

Product Description

GeneTex Mitochondria Isolation Kit (For Mammalian Cells And Tissues) is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the protein isolation procedure is simple, easy and user friendly with high yield. Unlike many commercial mitochondria preparation kits, this kit offers wide range of starting cells (5-50 million/sample) and intact mitochondria are isolated. The buffers are detergent and EDTA free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in less than 30 min.

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Enzyme Assay Assay dependent
Not tested in other applications.




For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
GTX35191_20180725_WB_Mitochondria Isolation Kit_18121410_388.jpg

GTX35191 WB Image

HepG2 cells were lysed and extracted by GTX35191 Mitochondria Isolation Kit .
When following the protocol, cell extracts would be isolated into cytosolic and total membrane.
The fractions were then assayed by Western Blot analysis, membrane was blotted with an organelle membrane protein- ATP5D antibody (GTX101503, subcellular marker) and beta Catenin antibody (GTX101435, internal control).
WCE : whole cell extract
CE : cytosolic extract
ME: total membrane extract