GeneTex
United States (US)

Trident Nuclear Protein Extraction Kit

GTX16374_WB_18121410_613.jpg

Cat No. GTX16374

Application WB
APPLICATION

Application Note

1. Read the entire protocol carefully.
2. Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, the addition of protease inhibitors to extracted lysate buffer is recommended.
3. The nuclear extraction buffer contains 300 mM salt, for some applications, dilution or desalting of the extract may be needed.
4. The capacity of protein extraction filter cartridge is 500 μL. Multiple filter cartridges can be used if larger amount of cell lysate is processed.
5. To study protein phosphorylation, phosphatase inhibitors should be added to lysis buffer prior to use.
PROPERTIES

Form

Liquid

Storage

Store at 4ºC

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Spin column-based protein extraction and cell fractionation technologies were developed by Invent Biotechnologies, Inc.
TARGET

Background

Trident Nuclear protein extraction kit is for fast extraction of native cytoplasmic and nuclear proteins from cultured mammalian cells or mammalian tissues. The procedure can be completed in less than 15 min. Extracted native cytoplasmic and nuclear proteins can be used for SDS-PAGE, immunoblottings, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels etc.
DATA IMAGES
GTX16374_WB_18121410_613.jpg

GTX16374 WB Image

293T cells' cytoplasmic and nuclear proteins were extracted by 8-min. Cytoplasmic & Nuclear Protein Extraction Kit (Cat.# GTX16374).Courtesy of a GeneTex collaborating partner
A. SDS-PAGE gel shows the profiles of extracted cytoplasmic proteins and nuclear proteins.
B. The proteins from the gel were transferred to nitrocellulose membrane and probed with Histone deacetylase 1 (HDAC1) antibody (nuclear marker) and Actin antibody (cytoplasmic marker).

Package List Price ($)
$ 349
$ 109