GeneTex
United States (US)

Trident RIPA Lysis Buffer

Trident RIPA Lysis Buffer. GTX400005
Trident RIPA Lysis Buffer. GTX400005

Cat No. GTX400005

Application WB
PRODUCT

Product Description

Radio-Immunoprecipitation assay lysis buffer
The ingredients of RIPA lysis buffer are as follows:
25 mM Tris pH 7.6
150 mM NaCl
1% Triton X-100
1% sodium deoxycholate
1% SDS
APPLICATION

Application Note

This product contains 1% SDS, which is higher than what is in some other commercial RIPA lysis buffers, and should facilitate a more thorough extraction of nuclear protein. The sample may be viscous following extraction due to unsheared DNA. To decrease this viscosity, we recommend increasing the volume of GTX400005 and trying either sonication or drawing the sample back-and-forth gently (to avoid bubbles) ten times through an 18G blunt needle attached to a 1ml syringe.
PROPERTIES

Form

Liquid

Storage

Storage Instruction: Store at 2-8 ºC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

RIPA lysis buffer is used to efficiently lyse most adherent and non-adherent mammalian cells. The extraction of cytosolic, membrane and nuclear proteins can be achieved by using GTX 400005 RIPA Lysis buffer. As the reagent would not adversely affect the antibodies and protein antigens, it often can be used for immunoprecipitation assay.
DATA IMAGES
Trident RIPA Lysis Buffer. GTX400005

GTX400005 WB Image

A431 and HeLa cells were lysed by using GTX400005 RIPA lysis buffer.
Whole cell extracts (30 μg) were assayed by Western Blot analysis.
The membrane was blotted with a cytosolic protein GAPDH antibody (GTX627408), a membrane protein Flotillin1 antibody (GTX104769) and a nuclear protein LaminB2 (GTX628803).

Trident RIPA Lysis Buffer. GTX400005

GTX400005 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

REFERENCE
Package List Price ($)
$ 59