GeneTex
United States (US)

Trident Universal Protein Blocking Reagent (animal serum free)

Cat No. GTX30963

Application WB, ICC/IF, IHC-P, ELISA, IHC
PRODUCT

Product Description

Protein Blocking Reagent (animal serum free)
APPLICATION

Application Note

Ready to use. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.
PROPERTIES

Form

Liquid

Buffer

20mM Tris 150mM, sodium chloride, 0.02% Triton X100, pH 7.4, and 0.5% Proclin 300 was added as a preservative. It does not contain SDS or sodium azide or any other preservative.

Storage

2-8ºC

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Blocking Solution, Blocking Buffer

Background

Immunohistochemistry (IHC)/ Immunocytochemistry (ICC) is the localization of antigens by the use of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used: labeled biotin secondary antibody streptavidin-peroxidase (LBSASP), HRP anti-HRP, ABC, catalyzed signal amplification, polymer system and others, to detect antigens on tissue and cell In this kit the first layer is unlabeled primary antibody, the second layer is biotinylated secondary antibody, the third layer is Enzyme-Streptavidin conjugate (HRP-Streptavidin) to replace the complex of avidin-biotin peroxidase. The enzyme is then visualized by application of the substrate chromogen solution to produce different colorimetric end products.

This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays.

REFERENCE
Package List Price ($)
$ 69