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Trident Universal Protein Blocking Reagent (animal serum free)

Cat. No. GTX30963

Reference 10

Application

 WB, ICC/IF, ELISA, IHC
Package
100 ml ($89)
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PRODUCT

Summary

Protein Blocking Reagent (animal serum free)

APPLICATION

Application Note

Ready to use. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.

PROPERTIES

Form

Liquid

Buffer

20mM Tris 150mM pH7.4, Sodium Chloride, 0.02% Triton X-100

Preservative

0.05% ProClin 300

Storage

Store at 2-8ºC.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Blocking Solution , Blocking Buffer

Background

Immunohistochemistry (IHC)/ Immunocytochemistry (ICC) is the localization of antigens by the use of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used: labeled biotin secondary antibody streptavidin-peroxidase (LBSASP), HRP anti-HRP, ABC, catalyzed signal amplification, polymer system and others, to detect antigens on tissue and cell In this kit the first layer is unlabeled primary antibody, the second layer is biotinylated secondary antibody, the third layer is Enzyme-Streptavidin conjugate (HRP-Streptavidin) to replace the complex of avidin-biotin peroxidase. The enzyme is then visualized by application of the substrate chromogen solution to produce different colorimetric end products.

This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays.

REFERENCE

Application Reference

Yi-Fan Chen et al. J Med Chem 2022; 65 (6) : 4767-4782
Shih HJ et al. Sci Rep 2021; 11 (1) : 22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Huang CM et al. Biomedicines 2021; 9 (10) miRNA-148a Enhances the Treatment Response of Patients with Rectal Cancer to Chemoradiation and Promotes Apoptosis by Directly Targeting c-Met.
Eakin AJ et al. Front Cell Dev Biol 2020; 8 : 799 Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function.
Connor KL et al. JCI Insight 2020; 5 (24) Identifying cell-enriched miRNAs in kidney injury and repair.
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REVIEW

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SDS
Proclin.pdf
Sodium Azide.pdf
Package List Price ($)
$ 89