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TrxR1 antibody

Anti-TrxR1 antibody used in Western Blot (WB). GTX110589
Anti-TrxR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110589
Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

Cat No. GTX110589

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A431 , mouse liver

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952462

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR

Cellular Localization

Cytoplasm , Isoform 4: Cytoplasm , Nucleus , Isoform 5: Cytoplasm

Background

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

GTX110589 WB Image

TrxR1 antibody detects TXNRD1 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
TrxR1 antibody (GTX110589) dilution: 1:1000

Anti-TrxR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110589

GTX110589 IHC-P Image

TrxR1 antibody detects TXNRD1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
TrxR1 antibody (GTX110589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

GTX110589 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX110589 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf