Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B(GTX100416) antibody at 1:500 dilution.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX100416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

UBE2B antibody detects UBE2B protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells
12 % SDS-PAGE
UBE2B antibody (GTX100416) dilution: 1:5000