Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX104717 diluted at 1:1000

Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 μg of UBE2L3 antibody (GTX104717).
Western blot analysis was performed using UBE2L3 antibody (GTX104717) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(GTX104717) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

UBE2L3 antibody detects UBE2L3 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
15 % SDS-PAGE
UBE2L3 antibody (GTX104717) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(GTX104717) antibody at 1:200 dilution.