10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide
Store at 2-8ºC.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Fluorescein isothiocyanate (FITC)
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
UAE, Ulex europaeus, UAE I Lectin, Fucose lectin, UAE I, Ulex europaeus lectin, UAE Lectin
The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.Inhibiting/Eluting sugars: 50-100 mM L-fucoseCarbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R