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UHRF1 antibody

KO/KD Orthogonal
Anti-UHRF1 antibody used in Immunoprecipitation (IP). GTX113963
Anti-UHRF1 antibody used in Western Blot (WB). GTX113963
Anti-UHRF1 antibody used in Immunoprecipitation (IP). GTX113963
Anti-UHRF1 antibody used in ChIP assay (ChIP assay). GTX113963
Anti-UHRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113963
Anti-UHRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113963
Anti-UHRF1 antibody used in Western Blot (WB). GTX113963
Anti-UHRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113963
Anti-UHRF1 antibody used in Western Blot (WB). GTX113963
Anti-UHRF1 antibody used in Western Blot (WB). GTX113963
Anti-UHRF1 antibody used in Western Blot (WB). GTX113963

Cat. No. GTX113963

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UHRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human UHRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin like with PHD and ring finger domains 1 , ICBP90 , Np95 , RNF106 , TDRD22 , hNP95 , hUHRF1 , huNp95

Cellular Localization

Nucleus

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UHRF1 antibody used in Immunoprecipitation (IP). GTX113963

GTX113963 IP Image

UHRF1 antibody immunoprecipitates UHRF1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 μg of UHRF1 antibody (GTX113963) 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by UHRF1 antibody (GTX113963) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-UHRF1 antibody used in Western Blot (WB). GTX113963

GTX113963 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody (GTX113963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UHRF1 antibody used in Immunoprecipitation (IP). GTX113963

GTX113963 IP Image

Immunoprecipitation of UHRF1 protein from HeLa whole cell extract using 5 μg of UHRF1 antibody (GTX113963).
Western blot analysis was performed using UHRF1 antibody (GTX113963).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-UHRF1 antibody used in ChIP assay (ChIP assay). GTX113963

GTX113963 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.

Anti-UHRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113963

GTX113963 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using UHRF1(GTX113963) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-UHRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113963

GTX113963 IHC-P Image

UHRF1 antibody detects UHRF1 protein at on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
UHRF1 antibody (GTX113963) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UHRF1 antibody used in Western Blot (WB). GTX113963

GTX113963 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody (GTX113963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-UHRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113963

GTX113963 ICC/IF Image

UHRF1 antibody detects UHRF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UHRF1 stained by UHRF1 antibody (GTX113963) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-UHRF1 antibody used in Western Blot (WB). GTX113963

GTX113963 WB Image

Orthogonal

The data was published in the journal Nucleic Acids Res in 2015. PMID: 25765655

Anti-UHRF1 antibody used in Western Blot (WB). GTX113963

GTX113963 WB Image

Orthogonal

The data was published in the journal Nucleic Acids Res in 2015. PMID: 25765655

Anti-UHRF1 antibody used in Western Blot (WB). GTX113963

GTX113963 WB Image

KO/KD Orthogonal

The data was published in the journal Nucleic Acids Res in 2015. PMID: 25765655

REFERENCE

Application Reference

Fan X et al. PLoS Genet 2021; 17 (9) : e1009773 Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
Application : IHC-P
Gao Y et al. Cancer Lett 2021; 520 : 172-183 UHRF1 promotes androgen receptor-regulated CDC6 transcription and anti-androgen receptor drug resistance in prostate cancer through KDM4C-Mediated chromatin modifications.
Application : ChIP assay
Kim KB et al. Nucleic Acids Res 2015; 43 (7) : 3509-23 H3K9 methyltransferase G9a negatively regulates UHRF1 transcription during leukemia cell differentiation.
Application : WB
Reactivity : Human
Lopez-Contreras AJ et al. Cell Rep 2013; 3 (4) : 1105-16 A proteomic characterization of factors enriched at nascent DNA molecules.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

UHRF1 antibody Cat. No. GTX113963

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-UHRF1 antibody used in Western Blot (WB). GTX113963
Date : Anonymous submitted on 20-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 40μg
Blocking : 5% skim milk in TBST, 22°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169